Campus Box 1134
The goal of our photosynthesis research is to achieve a molecular-level understanding of the primary charge separation process in the bacterial reaction center. In this pigment-protein complex, light energy is converted into chemical potential energy by a series of fast electron transfers across the membrane from the photoexcited bacteriochlorophyll special dimer along a chain of electron acceptors on the photoactive A-branch with a quantum yield of ~1. We have been preparing and studying mutants to (1) probe the contributions of energetics versus electronic interactions to the directionality of electron transfer, (2) modulate the rate constants and yields of charge separation versus recombination at each step on both branches, and (3) give electron transfer fully down the normally inactive B branch.
Our studies of tetrapyrrole chromophores (porphyrin, chlorin, bacteriochlorin) chromophores are aimed at elucidating the fundamental interplay between molecular composition, electronic structure, and photophysical properties. These properties include absorption and emission spectra and the rate constants and yields of the singlet excited-state decay pathways (fluorescence, internal conversion, intersystem crossing). Studies of multichromophore arrays probe fundamental aspects of energy and electron transfer and include design and characterization of biohybrid light-harvesting architectures that integrate designer chromophores with native-like peptide scaffolds to enhance solar coverage.
2007-present, Member, Imaging Sciences Pathway
2005-2013, Member, CMI
2013- present, Member, IMSE
1989-present, Professor, Department of Chemistry, Washington University
1986-1989, Associate Professor, Departmentof Chemistry, Washington University
1980-present, Member, DBBS
1980-1986, Assistant Professor, Department of Chemistry, Washington University
1977-1980, Postdoctoral Fellow, University of Washington, Seattle
Integration of Multiple Chromophores with Native Photosynthetic Antennas to Enhance Solar Energy Capture and Delivery, M. A. Harris, P. S. Parkes-Loach, J. W. Springer, J. Jiang, E. C. Martin, P. Qian, J. Jiao, D. M. Niedzwiedzki, C. Kirmaier, J. D. Olsen, D. F. Bocian, D. Holten, C. N. Hunter, J. S. Lindsey, and P. A. Loach Chemical Science 2013, 4, 3924–3933.
Distinct Photophysical and Electronic Characteristics of Strongly Coupled Dyads Containing a Perylene Accessory Pigment and a Porphyrin, Chlorin, or Bacteriochlorin, J. Wang, E.K. Yang, J. R. Diers, D. M. Niedzwiedzki, C. Kirmaier, D. F. Bocian, J. S. Lindsey, and D. Holten, J. Phys. Chem. B., 117, 9288–9304.
Amphiphilic Chlorins and Bacteriochlorins in Micellar Environments. MolecularDdesign, De Novo Synthesis, and Photophysical Properties, K. Aravindu, O. Mass, P. Vairaprakash, J. W. Springer, E. Yang, D. M. Niedzwiedzki, C. Kirmaier, D. F. Bocian, D. Holten, and J. S. Lindsey Chemical Science 2013, 4, 3459–3477.
Protein Influence on Charge-Asymmetry of the Primary Donor in Photosynthetic Bacterial Reaction Centers Containing a Heterodimer: Effects on Photophysical Properties and Electron Transfer, M. A. Harris, C. A. Luehr, K. M. Faries, M. Wander, L. Kressel, D. Holten, D. K. Hanson, P. D. Laible and C. Kirmaier J. Phys. Chem. B. 2013, 117, 4028-4041.
Palette of Lipophilic Bacteriochlorins for Construction of Biohybrid Light-Harvesting Architectures, K. R. Reddy, M. Krayer, M. A. Harris, J. W. Springer, E. Yang, D. M. Niedzwiedzki, C. Kirmaier, J. Jiao, P. S. Parkes-Loach, P. A. Loach, D. F. Bocian, D. Holten, and J. S. Lindsey Chemical Science 2013, 4, 2036-2053.
Photophysical Properties and Electronic Structure of Bacteriochlorin–Chalcones with Extended Near-Infrared Absorption, E. Yang, C. Ruzié, M. Krayer, J. R. Diers, D. M. Niedzwiedzki, C. Kirmaier, J. S. Lindsey, D. F. Bocian, and D. Holten Photochem. Photobiol. 2013, 89, 586-604.
Molecular Electronic Tuning of Photosensitizers to Enhance Photodynamic Therapy: Synthetic Dicyanobacteriochlorins as a Case Study, E. Yang, J. R. Diers, Y.-Y. Huang, K. Aravindu, M. R Hamblin, J. S Lindsey, D. F. Bocian, and D. Holten Photochem. Photobiol. 2013, 89, 605-818.
Photochemistry of a Bacterial Reaction Center Missing the Initial Bacteriochlorophyll Electron Acceptor, B. Carter, S. G. Boxer, D. Holten and C. Kirmaier J. Phys. Chem. B. 2012, 116, 9971-9982.
Biohybrid Photosynthetic Antenna Complexes for Enhanced Light-Harvesting, J. W. Springer, P. S. Parkes-Loach, K. R. Reddy, M. Krayer, J. Jiao, G. M. Lee, D.M. Niedzwiedzki, M. A. Harris, C. Kirmaier, D. F. Bocian, J. S. Lindsey, D. Holten, and P. A. Loach J. Am. Chem. Soc. 2012, 134, 4589-4599.High-Throughput Engineering to Revitalize a Vestigial Electron Transfer Pathway in Bacterial Photosynthetic Reaction Centers, K. M. Faries, L. L. Kressel, M. J. Wander, D. Holten, P. D. Laible, C. Kirmaier, and D. K. Hanson J. Biol. Chem. 2012, 287, 8507-8514.
2009, Outstanding Mentor Award, Washington University Graduate Student Senate
1999, Midwest Award (for Achievement in Chemistry, St. Louis Section of the ACS)
1991, Washington University Morter Board Teacher of the Month (November)
1989, St. Louis Award (St. Louis Section of the American Chemical Society)
1980, Camille and Henry Dreyfus Award for Newly Appointed Young Faculty in Chemistry
1977, NSF National Needs Postdoctoral Fellow
Chemistry 111, General Chemistry
Chemistry 151 General Chemistry Lab I
Chemistry 152 General Chemistry Lab II
Chemistry 442, Quantum Chemistry
Chemistry 445, Instrumental Methods in Physical Chemistry Laboratory
Chemistry 574, Optical Properties of Molecules