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On August 11, 2008, we labeled a water-stressed
soybean leaf with 400-ppm 13CO2 and 21% 17O2
(and the balance, N2). The goal was to discover the fate
of glycine produced by the oxygenase pathway of Rubisco, ribulose
bisphosphate carboxylase-oxygenase. There was 1 liter (STP) of labeling
gas purchased from Isotec (Miamisburg, OH) at a cost of $7,000.
The labeling lasted 20 minutes. The labeled leaf was frozen, lyophilized,
and examined by solid-state NMR.
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