Tentative Lecture and Exam Schedule for Chem 520, 2003.

Date

Class

Topic

January 13

1

Introduction, nomenclature, structure, properties of nucleotides

15 

2

Conformational analysis of nucleotides 

17 

3

Structure of A, B, Z DNA, triplexes and quadruplexes, tRNA

20

4

Thermodynamics of DNA duplex formation and RNA folding

22

5

Chemical and spectroscopic probes of DNA structure and interactions

25

6

Small molecule interactions: Intercalation and minor groove binding

27

7

DNA-protein interactions

29

8

DNA supercoiling

31

9

DNA bending  (Exam I)

February 3

10

Chemical synthesis of nucleotides, solution phase DNA synthesis

5

11

Automated DNA and RNA synthesis

7

12

2D synthesis, synthesis of circular NA, and modified NA (PNA)

10

13

Enzymatic synthesis of nucleotides, de novo, salvage

 

14

Enzymatic synthesis of DNA, RNA, and protein

 

15

Physical, chemical, and enzymatic sequencing of DNA and RNA

17

16

In vitro evolution, SELEX, catalytic DNA

 

17

Genetic engineering, site-directed mutagenesis

 

18

Protein expression, unnatural mutagenesis, semi-synthetic proteins (Exam II)

 24

19

Photochemistry of DNA

26

20

Radiation Chemistry

28

21

Radiomimetic Agents, Footprinting and affinity cleaving

March 10

22

Covalent Binders/Xenobiotics

12

23

DNA Repair

14

24

RNA cleavage chemistry (Exam III)

17

25

 

19

26

 

 21

27

 

 24

28

 

26

29

 

28

30

 

 31

31

 

April 2

32

 

4

33

 

7

34

 

9

35

 

11

36

 

14

37

 

16

38

 

18

39

 

21

40

 

23

41

 

25

42

 


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